The Application of Botanical Pesticides to Control Fusarium Wilt on Asparagus Beans

##plugins.themes.academic_pro.article.sidebar##

Published Feb 24, 2024
https://doi.org/10.55043/jaast.v8i1.190
Download
PDF
Statistic
Vol. 8 No. 1 (2024): Journal of Applied Agricultural Science and Technology

##plugins.themes.academic_pro.article.main##

Muh. Adiwena
Universitas Borneo Tarakan
https://orcid.org/0000-0002-5688-5747
Aditya Murtilaksono
Universitas Borneo Tarakan
https://orcid.org/0000-0001-5271-2024
Mikael Ngau
Universitas Borneo Tarakan
Rachmadaniar Rachmadaniar
Universitas Borneo Tarakan
Saat Egra
Gifu University
https://orcid.org/0000-0002-3259-7134
Ankardiansyah Pandu Pradana
Universitas of Jember
https://orcid.org/0000-0002-5706-5580
Ahmed Ibrahim Alrashid Yousif
Hungarian University of Agriculture and Life Science

Abstract

Triggered by a fungus of the genus Fusarium, wilting prevails in various horticultural crops in Indonesia. In asparagus beans, this pathogen can lead to decreased production. One eco-friendly controlling measure is the application of botanical pesticides made from betel leaves and neem leaves which have been extensively used to control various pathogenic fungi.  This study aims to evaluate the effectiveness of plant-based pesticides made from neem and betel leaves in controlling Fusarium wilt in asparagus beans. This research was run for approximately six months, initiated at the Laboratory of Plant Protection in Universitas Borneo Tarakan, and ended in the Experimental Garden of the Faculty of Agriculture. It involved Fusarium propagation, preparation of planting media, planting asparagus beans, infecting Fusarium, and producing as well as applying botanical pesticides. It employed a one-factor randomized block design with 5 treatments, control (P0), 5% neem leaf extract per plant (P1), 10% neem leaf extract per plant (P2), 5% betel leaf extract per plant (P3) and 10% betel leaf extract per plant (P4). The covariance analysis results showed that the treatment did nothave a significant effect on the number of affected branches, the number of pods and pod weight. Even so, it can be concluded that P2 can reduce wilted branches by up to 48%, with the same concentration, and P4 can reduce wilted branches by up to 52%. In terms of the total number and the total weight of the pods, P4 achieved the best results with 77 pods, weighing 3,272 grams.

##plugins.themes.academic_pro.article.details##

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Author Biographies

Muh. Adiwena, Universitas Borneo Tarakan

Department of Agrotechnology

Aditya Murtilaksono, Universitas Borneo Tarakan

Department of Agrotechnology

Mikael Ngau, Universitas Borneo Tarakan

Department of Agrotechnology

Rachmadaniar Rachmadaniar, Universitas Borneo Tarakan

Department of Agrotechnology

Saat Egra, Gifu University

United Graduate School of Agriculture Sciences

Ankardiansyah Pandu Pradana, Universitas of Jember

Department of Plant Protection

How to Cite
Adiwena, M. ., Murtilaksono, A., Ngau, M. ., Rachmadaniar, R., Egra, S., Pradana, A. P. ., & Yousif, A. I. A. . (2024). The Application of Botanical Pesticides to Control Fusarium Wilt on Asparagus Beans. Journal of Applied Agricultural Science and Technology, 8(1), 1-11. https://doi.org/10.55043/jaast.v8i1.190

References

  1. Agustin, S., Asrul, A., & Rosmini, R. (2016). Efektivitas Ekstrak Daun Mimba (Azadirachta indica A. Juss) terhadap Pertumbuhan Koloni Alternaria Porri Penyebab Penyakit Bercak Ungu pada Bawang Wakegi (Allium x wakegi ArakiSecara In Vitro. Agrotekbis: E-Jurnal Ilmu Pertanian, 4(4), 419–424. http://jurnal.faperta.untad.ac.id/index.php/agrotekbis/article/view/40
  2. Ali, A., Shahid, M. A., Hossain, M. D., & Islam, M. N. (2019). Antibacterial Bi-Layered Polyvinyl Alcohol (PVA)-Chitosan Blend Nanofibrous Mat Loaded with Azadirachta indica (neem) extract. International Journal of Biological Macromolecules, 138, 13–20. https://doi.org/10.1016/j.ijbiomac.2019.07.015
  3. Arsih, D. W., Panggeso, J., & Lakani, I. (2015). Uji Ekstrak Daun Sirih dan Cendawan Trichoderma sp dalam menghambat perkembangan Fusarium oxysporum f.Sp lycopersici Penyebab Penyakit Layu Fusarium pada Tanaman Tomat. Natural Science: Journal of Science and Technology, 4(3), 355–368. https://doi.org/10.22487/25411969.2015.v4.i3.5141
  4. Banerjee, K., Gadani, M. H., Srivastava, K. K., Verma, N., Jasrai, Y. T., & Jain, N. K. (2013). Screening of Efficient Arbuscular Mycorrhizal Fungi for Azadirachta indica under Nursery Condition: A Step Towards Afforestation of Semi-Arid Region of Western India. Brazilian Journal of Microbiology, 44(2), 587–594. https://doi.org/10.1590/S1517-83822013005000046
  5. Cahyaningrum, H., Prihatiningsih, N., & Soedarmono, S. (2017). Intensitas dan Luas Serangan Beberapa Isolat Fusarium oxysporum f.Sp. Zingiberi pada Jahe Gajah. Jurnal Perlindungan Tanaman Indonesia, 21(1), 16–22. https://doi.org/10.22146/jpti.17743
  6. Dewi, A. A. L. N., Karta, I. W., Wati, N. L. C., & Dewi, N. M. A. (2017). Uji Efektivitas Larvasida Daun Mimba (Azadirachta indica) terhadap Larva Lalat Sarcophaga pada Daging untuk Upakara yadnya di Bali. JST (Jurnal Sains dan Teknologi), 6(1), 126–135. https://doi.org/10.23887/jstundiksha.v6i1.9233
  7. Di, X., Takken, F. L., & Tintor, N. (2016). How Phytohormones Shape Interactions Between Plants and The Soil-Borne Fungus Fusarium oxysporum. Frontiers in Plant Science, 7(170), 1–9. https://doi.org/10.3389/fpls.2016.00170
  8. Doehlemann, G., & Hemetsberger, C. (2013). Apoplastic Immunity and It’s Suppression by Filamentous Plant Pathogens. New Phytologist, 198(4), 1001–1016. https://doi.org/10.1111/nph.12277
  9. Efendi, E. (2016). Implementasi Sistem Pertanian Berkelanjutan dalam Mendukung Produksi Pertanian. Warta Dharmawangsa, (47). https://doi.org/10.46576/wdw.v0i47.231
  10. Egbuta, M. A., Mwanza, M., & Babalola, O. O. (2016). A Review of The Ubiquity of Ascomycetes Filamentous Fungi in Relation to Their Economic and Medical Importance. Advances in Microbiology, 6(14), 1140-1158. https://www.scirp.org/pdf/AiM_2016122815152735.pdf
  11. Everts, K. L., Egel, D. S., Langston, D., & Zhou, X.-G. (2014). Chemical Management of Fusarium Wilt of Watermelon. Crop Protection, 66, 114–119. https://doi.org/10.1016/j.cropro.2014.09.003
  12. Fitriani, M. L., Wiyono, S., & Sinaga, M. S. (2019). Potensi Kolonisasi Mikoriza Arbuskular dan Cendawan Endofit dan Kemampuannya dalam Pengendalian Layu Fusarium pada Bawang Merah. Jurnal Fitopatologi Indonesia, 15(6), 228–238. https://doi.org/10.14692/jfi.15.6.228-238
  13. Ganul, T., Alang, H., & Ainulia, A. D. R. (2021). Pengaruh Ekstrak Rimpang Jeringau (Acorus calamus L) terhadap Larva Ulat Grayak (Spodoptera litura) pada Sawi Putih (Brassica pekinensis). Celebes Biodiversitas: Jurnal Sains dan Pendidikan Biologi, 4(1), 6–16. https://doi.org/10.51336/cb.v4i1.255
  14. Gholamnezhad, J. (2019). Effect of Plant Extracts on Activity of Some Defense Enzymes of Apple Fruit in Interaction with Botrytis cinerea. Journal of Integrative Agriculture, 18(1), 115–123. https://doi.org/10.1016/S2095-3119(18)62104-5
  15. Gita, R. S. D., & Danuji, S. (2021). Studi Keanekaragaman Tumbuhan Obat yang Digunakan dalam Pengobatan Tradisional Masyarakat Kabupaten Pamekasan. Bioma: Jurnal Biologi dan Pembelajaran Biologi, 6(1), 11–23. https://doi.org/10.32528/bioma.v6i1.4817
  16. Grdiša, M., & Gršić, K. (2013). Botanical Insecticides in Plant Protection. Agriculturae Conspectus Scientificus, 78(2), 85–93. https://hrcak.srce.hr/104637
  17. Hartati, S. S., Rustiani, U. S., Puspasari, L. T., & Kurniawan, W. (2016). Kompatibilitas Vegetatif Fusarium oxysporum dari Beberapa Tanaman Inang. Jurnal Agrikultura, 27(3). https://doi.org/10.24198/agrikultura.v27i3.10875
  18. Hashmat, I., Azad, H., & Ahmed, A. (2012). Neem (Azadirachta indica A. Juss)-A Nature’s Drugstore: An Overview. International Research Journal of Biological Sciences, 1(6), 76–9. http://www.isca.me/IJBS/Archive/v1/i6/14.ISCA-IRJBS-2012-150.php
  19. Henry, P. M., Haugland, M., Lopez, L., Munji, M., Watson, D. C., & Gordon, T. R. (2020). The Potential for Fusarium oxysporum f.Sp. Fragariae, cause of Fusarium Wilt of Strawberry, to Colonize Organic Matter in Soil and Persist Through Anaerobic Soil Disinfestation. Plant Pathology, 69(7), 1218–1226. https://doi.org/10.1111/ppa.13225
  20. Juariyah, S., Tondok, E. T., & Sinaga, M. S. (2018). Trichoderma dan Gliocladium untuk Mengendalikan Penyakit Busuk Akar Fusarium pada Bibit Kelapa Sawit. Jurnal Fitopatologi Indonesia, 14(6), 196–204. https://doi.org/10.14692/jfi.14.6.196
  21. Kakouridis, A., Hagen, J. A., Kan, M. P., Mambelli, S., Feldman, L. J., Herman, D. J., Weber, P. K., Peet-Ridge, J., & Firestone, M. K. (2022). Routes to Roots: Direct Evidence of Water Transport by Arbuscular mycorrhizal Fungi to Host Plants. New Phytologist, 236(1), 210–221. https://doi.org/10.1111/nph.18281
  22. Keter, R. J. (2012). Macroscopic and Microscopic Study of Datura stramonium, Azadirachta indica and Bidens Pilosa [Doctoral Dissertation]. Nairobi: University of Nairobi. http://erepository.uonbi.ac.ke/handle/11295/74506
  23. Kishore, G. K., & Pande, S. (2005). Natural Fungicides for Management of Phyto-Pathogenic Fungi. In Lal, A., & Gour, H. N., (Eds). Annual Review of Plant Pathology, (pp. 331–356). Scientific Publishers.
  24. Kurniawan, B. (2019). Pengawasan Pengelolaan Limbah Bahan Berbahaya dan Beracun (B3) di Indonesia dan Tantangannya. Dinamika Governance: Jurnal Ilmu Administrasi Negara, 9(1), 39–49. https://doi.org/10.33005/jdg.v9i1.1424
  25. Mudmainah, S., & Khatimah, K. (2022). Pengaruh Aplikasi Pemberian Pupuk NPK terhadap Produksi dan Perkembangan Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Melon (Cucumis melo L.) di Rumah Kaca: Array. Jurnal Pertanian Peradaban (Peradaban Journal of Agriculture), 2(1), 36–45. https://journal.peradaban.ac.id/index.php/jpp/article/view/976
  26. Naja, A. P., Andika, & Mi’rajunnisa. (2022). Studi In Silico Senyawa Metabolit Sekunder Daun Sirih Hijau (Piper betle L) sebagai Inhibitor Enzim Main Protease (Mpro) pada SARS-CoV-2. Medical Sains: Jurnal Ilmiah Kefarmasian, 7(2), 343–356. https://doi.org/10.37874/ms.v7i2.337
  27. Nurhasanah, N. (2020). Uji Dosis Fungisida Berbahan Aktif Propineb dan Waktu Aplikasi Terhadap Pertumbuhan (Fusarium oxysporum) Secara In Vitro [Thesis]. Riau: Universitas Islam Riau. https://repository.uir.ac.id/8750/
  28. Odebode, A., Adekunle, A., Stajich, J., & Adeonipekun, P. (2020). Airborne Fungi Spores Distribution in Various Locations in Lagos, Nigeria. Environmental monitoring and assessment, 192(87), 1–14. https://doi.org/10.1007/s10661-019-8038-3
  29. Perincherry, L., Lalak-Kańczugowska, J., & Stępień, Ł. (2019). Fusarium-Produced Mycotoxins in Plant-Pathogen Interactions. Toxins, 11(11), 664. https://doi.org/10.3390/toxins11110664
  30. Ploetz, R. C. (2006). Fusarium Wilt of Banana is Caused by Several Pathogens Referred to as Fusarium oxysporum f.Sp. Cubense. Phytopathology, 96(6), 653–656. https://doi.org/10.1094/PHYTO-96-0653
  31. Rahayu, L. A. (2015). Identifikasi dan Deskripsi Fungi Penyebab Penyakit pada Tanaman Kacang Panjang (Vigna sinensis L.) [Thesis]. Jakarta: UIN Syarif Hidayatullah Jakarta. https://repository.uinjkt.ac.id/dspace/handle/123456789/27805
  32. Rahmat, R. A. (2020). Potensi Formulasi Pasta Gigi Kombinasi Ekstrak Etanol Daun Sirih (Piper Betle L.), Biji Pinang (Areca Catechu), Gambir (Uncaria Gambir) dalam Menghambat Pertumbuhan Bakteri Staphylococcus Aureus [Thesis]. Surakarta: Universitas Muhammadiyah Surakarta. https://eprints.ums.ac.id/85858/
  33. Ruliyanti, W., & Majid, A. (2020). Pengaruh Pemberian Vermikompos pada Media Tanam Terhadap Efektivitas Gliocladium sp. dalam Mengendalikan Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Semangka (Citrulus vulgaris, Schard). Jurnal Pengendalian Hayati, 3(1), 14–21. https://doi.org/10.19184/jph.v3i1.17147
  34. Singh, R., Biswas, S. K., Nagar, D., Singh, J., Singh, M., & Mishra, Y. K. (2015). Sustainable Integrated Approach for Management of Fusarium Wilt of Tomato caused by Fusarium oxysporum f.Sp. Lycopersici (Sacc.) Synder and Hansen. Sustainable Agriculture Research, 4(1), 138–147. http://dx.doi.org/10.5539/sar.v4n1p138
  35. Suanda, I. W., & Sumarya, I. M. (2021). Ekstraksi dan Fraksinasi Daun Brotowali (Tinospora crispa L. Miers) untuk Insektisida Nabati pada Larva Plutella xylostella L. Jurnal Widya Biologi, 12(01), 17–33. https://doi.org/10.32795/widyabiologi.v12i01.1320
  36. Sudantha, I. M., & Abad, A. L. (2018). Uji Efektivitas Beberapa Jenis Jamur Endofit Trichoderma spp. Isolat Lokal NTB terhadap Jamur Fusarium oxysporum f.Sp. Vanillae Penyebab Penyakit Busuk Batang pada Bibit Vanili. Crop Agro, Jurnal Ilmiah Budidaya, 4(2), 64–73. https://www.cropagro.unram.ac.id/index.php/caj/article/view/103
  37. Suriani, Sebayang, A., Mirsam, H., Pakki, S., Azrai, M., & Muis, A. (2021). Control of Fusarium verticillioides on Corn with a Combination of Bacillus subtilis TM3 Formulation and Botanical Pesticides. Saudi Journal of Biological Sciences, 28(12), 7000–7005. https://doi.org/10.1016/j.sjbs.2021.07.083
  38. Sutrisni, R., & Widodo. (2012). Keragaman Fusarium pada Rizosfer Tanaman Kacang Panjang dan Peranannya bagi Pertumbuhan Tanaman. Jurnal Fitopatologi Indonesia, 8(5), 128–137. https://doi.org/10.14692/jfi.8.5.128
  39. Tampubolon, K., Sihombing, F. N., Purba, Z., Samosir, S. T. S., & Karim, S. (2018). Potensi Metabolit Sekunder Gulma sebagai Pestisida Nabati di Indonesia. Kultivasi, 17(3), 683–693. https://doi.org/10.24198/kultivasi.v17i3.18049
  40. Vu, D., Groenewald, M., De Vries, M., Gehrmann, T., Stielow, B., Eberhardt, U., Al-Hatmi, A., Groenewald, J. Z., Cardinali, G., Houbraken, J., Boekhout, T., Crous, P. W., Robert, V., & Verkley, G. J. M. (2019). Large-Scale Generation and Analysis of Filamentous Fungal DNA Barcodes Boosts Coverage for Kingdom Fungi and Reveals Thresholds for Fungal Species and Higher Taxon Delimitation. Studies in Mycology, 92(1), 135–154. https://doi.org/10.1016/j.simyco.2018.05.001
  41. Wahyu, A. S. (2021). Uji Konsentrasi Bahan Aktif Mankozeb dan Waktu Aplikasi terhadap Jamur (Fusarium Oxyporum) pada Tanaman Cabai Merah (Capsicum Annum L.) Secara In-vitro [Thesis]. Riau: Universitas Islam Riau. https://repository.uir.ac.id/17602/
  42. Wahyuni, N. M. D., Astiti, N. P. A., & Proborini, M. W. (2019). Identifikasi Senyawa Aktif Ekstrak Daun Tembelekan (Lantana camara L.) yang Berpotensi sebagai Pengendali Jamur Fusarium solani (Mart.) Sacc. Penyebab Layu Batang dan Busuk Akar Tanaman Pisang Kepok (Musa paradisiaca forma typica). Jurnal Metamorfosa, 6(2), 191–197. https://doi.org/10.24843/metamorfosa.2019.v06.i02.p04
  43. Wegulo, S. N., Baenziger, P. S., Nopsa, J. H., Bockus, W. W., & Hallen-Adams, H. (2015). Management of Fusarium Head Blight of Wheat and Barley. Crop Protection, 73, 100–107. https://doi.org/10.1016/j.cropro.2015.02.025
  44. Yi, U., Zaharah, S. S., Ismail, S. I., & Musa, M. H. (2021). Effect of Aqueous Neem Leaf Extracts in Controlling Fusarium Wilt, Soil Physicochemical Properties and Growth Performance of Banana (Musa spp.). Sustainability, 13(22), 12335. https://doi.org/10.3390/su132212335
  45. Yolanda, N. P. (2022). Uji Antibakteri Kombucha Daun Sirih (Piper betle L.) Terhadap Bakteri Propionibacterium acnes [Thesis]. Bandar Lampung: Universitas Islam Negeri Raden Intan Lampung. http://repository.radenintan.ac.id/17577/#
  46. Zhao, X., Li, H., Zhou, L., Chen, F., & Chen, F. (2020). Wilt of Acer negundo L. caused by Fusarium nirenbergiae in China. Journal of Forestry Research, 31(5), 2013–2022. https://doi.org/10.1007/s11676-019-00996-9
  47. Zhao, Z., Yang, X., Zhao, X., Chen, L., Bai, B., Zhou, C., & Wang, J. (2018). Method Development and Validation for The Analysis of Emerging and Traditional Fusarium Mycotoxins in Pepper, Potato, Tomato, and Cucumber by UPLC-MS/MS. Food Analytical Methods, 11, 1780–1788. https://doi.org/10.1007/s12161-018-1180-7