Investigation of the Effect of Heating Duration in Gambier (Uncaria gambir Roxb.) Processing on Its Phytochemical Composition and the Resulting Characteristics of a Gambier-based Acne Balm Product
##plugins.themes.academic_pro.article.main##
Abstract
Gambier (Uncaria gambir Roxb.) contains potent bioactive compounds with strong natural antimicrobial properties, making it a promising ingredient for acne treatment. However, its application in topical formulations remains limited due to a lack of understanding regarding the impact of processing conditions, particularly heating, on its functional efficacy. This study investigated the effect of heating duration on the characteristics of an acne treatment balm formulated with black cube gambier. Experiments using a completely randomized design (CRD) were carried out for 0, 30, 60, 90, and 120 minutes of heating durations to evaluate the balm's physical, chemical, and microbial properties. The results demonstrate that heating duration significantly influenced key parameters, including homogeneity, spreadability, melting point, irritation potential, texture, color, pH, total phenol content, and antibacterial activity (p < 0.05). The most optimal results were obtained at 120 minutes, yielding a balm with superior homogeneity, spreadability (4.47 cm), melting point (50°C), pH (7.44), total phenol content (54.28%), and diameter of the inhibition zone (16.35 mm). FTIR analysis confirmed an increase in phenolic compound stability, indicating enhanced antibacterial potential. These findings suggest that extended heating duration improves the stability and performance of gambier-based formulations. The optimized acne balm formulation highlights the potential of locally sourced gambier as a natural, eco-friendly acne treatment. Further research is recommended to evaluate product shelf-life, consumer acceptability, and industrial scalability.
##plugins.themes.academic_pro.article.details##

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
References
- Anggraini T, Syukri D. Uncaria gambir as the potential commodities from West Sumatra, Indonesia: A mini review. Asian J Plant Sci 2023;22:583-593. https://doi.org/10.3923/ajps.2023.583.593
- Efrina A, Kasim A, Aggraini T, Novelina, Asben A, Syukri D, et al. Utilization of Gambier (Uncaria gambier, Roxb.) combined with albumin in formation of natural rigid foam. J Glob Innov Agric Sci 2025;13:1-7. https://jgiass.com/pdf-reader.php?file=Utilization-of-Gambier-(Uncaria-Gambier,-Roxb.)-Combined-with-Albumin-in-Formation-of-Natural-Rigid-Foam.pdf&path=inpress.
- Syukri D, Rini R, Jessica A. Optimization of cathechin extraction for development of liquid hand soap made from gambier. Andalasian Int J Agric Nat Sci 2024;4:31-36. https://doi.org/10.25077/aijans.v4.i01.31-36.2023.
- Michalak M. Plant-Derived Antioxidants: Significance in Skin Health and the Ageing Process. Int J Mol Sci 2022;23:585. https://doi.org/10.3390/ijms23020585.
- Sun J, Jiang Y, Fu J, He L, Guo X, Ye H, et al. Beneficial Effects of Epigallocatechin Gallate in Preventing Skin Photoaging: A Review. Molecules 2024;29:5226. https://doi.org/10.3390/molecules29225226.
- Kapoor MP, Sugita M, Fukuzawa Y, Timm D, Ozeki M, Okubo T. Green Tea Catechin Association with Ultraviolet Radiation-Induced Erythema: A Systematic Review and Meta-Analysis. Molecules 2021;26:3702. https://doi.org/10.3390/molecules26123702.
- Mita SR, Muhtar NI, Kusuma SAF, Sriwidodo S, Hendrawan RP. Catechins as Antimicrobial Agents and Their Contribution to Cosmetics. Cosmetics 2025;12:11. https://doi.org/10.3390/cosmetics12010011.
- Huang Z, Lim JJ, Reginald K, Say YH, Chew FT. The Singapore/Malaysia Cross-Sectional Genetics Epidemiology Study: An Updated and Detailed Analysis of Acne Vulgaris Severity, Scarring, and Phenotypes and Their Associated Risk Factors among Young Chinese Adults. Dermatology 2025:1-15. https://doi.org/10.1159/000547009.
- Rahmadiawan D, Abral H, Iby IC, Kim HJ, Ryu K, Kwack HW, et al. Effect of post-heat treatment on the UV transmittance, hydrophobicity, and tensile properties of PVA/Uncaria gambir extract blend films. Heliyon 2024;10:e30748. https://doi.org/10.1016/j.heliyon.2024.e30748.
- Lapisa R, Fauza AN, Rahmadiawan D, Krismadinata, Yuvenda D, Putra RP, et al. The effect of Uncaria gambir on optical properties and thermal stability of CNF/PVA biocomposite films. J Renew Mater 2024;12:1-10. https://doi.org/10.32604/jrm.2024.053651.
- Kurniawan A, Dewi RM. Gambir (Uncaria gambir Roxb.) as a potential alternative treatment for hyperlipidemia. Jurnal Penelitian Kesehatan 2021;19:183-192. https://doi.org/10.22435/mpk.v31i3.4472.
- Melia S, Novia D, Juliyarsi I. Antioxidant and antimicrobial activities of gambir (Uncaria gambir Roxb.) extracts and their application in rendang. Pak J Nutr 2015;14:938-941. https://www.researchgate.net/publication/288686177_Antioxidant_and_Antimicrobial_Activities_of_Gambir_Uncaria_gambir_Roxb_Extracts_and_Their_Application_in_Rendang.
- Iskandar D, Rollando R, Susana S. Antioxidant and anti-breast cancer from Uncaria gambir Roxb leaves: In silico & in vitro study. Tradit Med J 2024;29:1-10. https://doi.org/10.22146/mot.92604.
- Franco-Gil ME, Graça A, Martins A, Marto J, Ribeiro HM. Emollients in dermatological creams: Early evaluation for tailoring formulation and therapeutic performance. Int J Pharm 2024;653:123825. https://doi.org/10.1016/j.ijpharm.2024.123825.
- Chen J, Ma Y, Tao Y, Zhao X, Xiong Y, Chen Z, et al. Formulation and evaluation of a topical liposomal gel containing a combination of zedoary turmeric oil and tretinoin for psoriasis activity. J Liposome Res 2021;31:130-144. https://doi.org/10.1080/08982104.2020.1748646.
- Coates J. Interpretation of Infrared Spectra, A Practical Approach. 2006. https://doi.org/10.1002/9780470027318.a5606.
- Mokari A, Guo S, Bocklitz T. Exploring the Steps of Infrared (IR) Spectral Analysis: Pre-Processing, (Classical) Data Modelling, and Deep Learning. Molecules 2023;28:6886. https://doi.org/10.3390/molecules28196886.
- Yang H, Yan R, Chen H, Lee DH, Zheng C. Characteristics of hemicellulose, cellulose and lignin pyrolysis. Fuel 2007;86:1781-1788. https://doi.org/10.1016/j.fuel.2006.12.013.
- Bolling JC, Duncan SE, Eigel WN, Waterman KM. Processing effects on physicochemical properties of creams formulated with modified milk fat. J Dairy Sci 2005;88:1342-1351. https://doi.org/10.3168/jds.S0022-0302(05)72800-9.
- Aguilar L, Hernández J, López-Giraldo LJ, Mercado R. Effect of Incorporating a Biowax Derived from Hydroprocessing of Crude Palm Oil in a Facial Cream and a Blemish Balm Cream. Cosmetics 2023;10:123. https://doi.org/10.3390/cosmetics10050123.
- Othmeni I, Karoui R, Blecker C. Impact of pH on the structure, interfacial and foaming properties of pea protein isolate: Investigation of the structure-function relationship. Int J Biol Macromol 2024;278:134818. https://doi.org/10.1016/j.ijbiomac.2024.134818.
- Dai J, Mumper RJ. Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties. Molecules 2010;15:7313-7352. https://doi.org/10.3390/molecules15107313.
- Djenane D, Miri YB, Ariño A. Use of Algerian Type Ras El-Hanout Spices Mixture with Marination to Increase the Sensorial Quality, Shelf Life, and Safety of Whole Rabbit Carcasses under Low-O2 Modified Atmosphere Packaging. Foods 2023;12:2931. https://doi.org/10.3390/foods12152931.
- Syukri D, Rini R, Fiana RM, Jonrinaldi, Primalita R, Aisyah Q, et al. Trends and innovations in sterilization of ready-to-eat food products: Challenges and future directions. OnLine J Biol Sci 2025;25:373-382. https://doi.org/10.3844/ojbsci.2025.373.382.
- Syukri D, Yenrina R, Putri MD, Darmawulan R, Refdi CW, Techavuthiporn C, et al. Metabolomic profiling of red chili (Capsicum annum L) puree stored in a fish-pond by gas chromatography-mass spectrometry (GC-MS). Asian J Green Chem 2025;9:231-245. https://doi.org/10.48309/AJGC.2025.491055.1602