Microbiological Stability of Traditional Rendang Boleces Prepared from Spent Hen Meat Under Ambient Storage Conditions
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Abstract
Spent hen meat has potential as an alternative raw material for traditional ready-to-eat meat products; however, its microbiological quality during storage requires evaluation. This study assessed the microbiological quality of rendang boleces (a meatball-like product) prepared from spent hen meat using Total Plate Count (TPC) analysis and Salmonella spp. detection. Samples were stored under ambient conditions (25–28°C) for 0, 2, 4, and 6 days. TPC values increased during storage, from 2.67 log CFU/g on day 0 to 4.63 log CFU/g on day 6. Samples stored for up to two days remained within the maximum permissible limit established by the Indonesian National Standard (SNI). In contrast, higher microbial counts were observed on days 4 and 6, indicating progressive microbial growth during extended storage. Salmonella spp. was not detected in any sample throughout the observation period. These findings indicate that rendang boleces maintained acceptable microbiological quality for up to two days under ambient storage conditions. The study also highlights the importance of appropriate storage and hygienic handling practices to maintain the quality of ready-to-eat meat products derived from spent hen meat while supporting the sustainable utilization of poultry resources in line with SDG 12 (Responsible Consumption and Production).
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References
- Fan H, Wu J. Conventional use and sustainable valorization of spent egg-laying hens as functional foods and biomaterials: a review. Bioresour Bioprocess 2022;9:43. https://doi.org/10.1186/s40643-022-00529-z
- Lee SH, Kim HY. Comparison of quality and sensory characteristics of spent hen and broiler in South Korea. Animals 2021;11:2565. https://doi.org/10.3390/ani11092565
- OECD, Food and Agriculture Organization of the United Nations (FAO). OECD-FAO agricultural outlook 2022–2031. Paris: OECD Publishing 2022. https://doi.org/10.1787/f1b0b29c-en
- Zarim NA, Abidin SZ, Ariffin F. Shelf life stability and quality study of texture-modified chicken rendang using xanthan gum as thickener for the consumption of the elderly with dysphagia. Food Biosci 2021;42:101054. https://doi.org/10.1016/j.fbio.2021.101054
- Sulieman AME, Abdallah EM, Alanazi NA, Ed-Dra A, Jamal A, Idriss H, et al. Spices as sustainable food preservatives: a comprehensive review of their antimicrobial potential. Pharmaceuticals 2023;16:1451. https://doi.org/10.3390/ph16101451
- Badan Standardisasi Nasional (BSN). Standar Nasional Indonesia (SNI 7388 : 2009) : Cemaran mikroba pada pangan olahan. Jakarta : BSN 2009. https://drive.google.com/file/d/1F5aOkqKKoFzFh9Bp_TwE11XN4E0JGw9n
- Patel A, Wolfram A, Desin TS. Advancements in detection methods for Salmonella in food: a comprehensive review. Pathogens 2024;13:1075. https://doi.org/10.3390/pathogens13121075
- European Food Safety Authority (EFSA), European Centre for Disease Prevention and Control (ECDC). The European Union One Health 2023 zoonoses report. EFSA J 2024;22:e9106. https://doi.org/10.2903/j.efsa.2024.9106
- Marmion M, Macori G, Whyte P, Scannell AG. Poultry abattoir site microbiome analysis reveals a core, time-independent processor microbiota. ACS Food Sci Technol 2023;3:2045–64. https://doi.org/10.1021/acsfoodscitech.3c00238
- Suryono S, Syarifuddin H, Maranata J, Musyid M, Nizori A. Development of high calcium chicken nuggets fortified with various citric acid extracted chicken eggshells powder. Journal of Applied Agricultural Science and Technology 2023;7:36–44. https://doi.org/10.55043/jaast.v7i1.133
- Anggarkasih MG, Febrinda AE, Adzkiya MA, Khasanah KA, Rahman RS, Maulani SP. Organoleptic Analysis, Quality Requirement, And Color Determination of Tilapia Nuggets with Eleutherine palmifolia Extract Coating. Journal of Applied Agricultural Science and Technology 2023;7:455–68. https://doi.org/10.55043/jaast.v7i4.151
- Söderqvist K, Peterson M, Johansson M, Olsson V, Boqvist S. A microbiological and sensory evaluation of modified atmosphere-packed (MAP) chicken at use-by date and beyond. Foods 2024;13:2140. https://doi.org/10.3390/foods13132140
- Augustyńska-Prejsnar A, Hanus P, Ormian M, Kačániová M, Sokołowicz Z, Topczewska J. The effect of temperature and storage duration on the quality and attributes of the breast meat of hens after their laying periods. Foods 2023;12:4340. https://doi.org/10.3390/foods12234340
- Weerasooriya G, Alwis HMTDPS, Bandara S, Premarathne KRPS, Liyanagunawardena NDN, Wijemuny MI et al. Persistence of Salmonella and Campylobacter on whole chicken carcasses under different chlorine concentrations used in the chill tank of processing plants in Sri Lanka. Pathogens 2024;13:664. https://doi.org/10.3390/pathogens13080664
- Drake MA, Watson ME, Liu Y. Sensory analysis and consumer preference: best practices. Annu Rev Food Sci Technol 2023;14:427–448. https://doi.org/10.1146/annurev-food-060721-023619
- Naumov K. The employment of standardized recipes, an essential prerequisite for consistent quality of the culinary product. In: ERAZ 2023: Knowledge-Based Sustainable Development—Conference Proceedings. Belgrade: Udruženje ekonomista i menadžera Balkana 2023;401–410. https://doi.org/10.31410/ERAZ.2023.401
- Roh T, Song Y, Yoon B. Integrated quality prediction model for food quality management based on Escherichia coli in shared kitchens. Foods 2024;13:4065. https://doi.org/10.3390/foods13244065
- Sari RP, Faridah A, Holinesti R, Mustika S. Analisis kualitas bakso ayam petelur afkir dengan penambahan karagenan sebagai bahan pengenyal. Jurnal Pendidikan Tata Boga dan Teknologi 2023;4:318–24. https://doi.org/10.24036/jptbt.v4i2.10986
- Ye Y, Chen F, Shi M, Wang Y, Xiao X, Wu C. Gel properties and protein structures of minced pork prepared with κ-carrageenan and non-meat proteins. Gels 2024;10:305. https://doi.org/10.3390/gels10050305
- Xia G, Jia R, Tong J, Zhang X, Zhang S, Wei H, et al. Cryoprotective effects of carrageenan on pre-prepared gel of minced shrimp (Litopenaeus vannamei) during frozen storage. Food Bioprocess Technol 2023;16:2082–94. https://www.researchgate.net/publication/369092096_Cryoprotective_Effects_of_Carrageenan_on_Pre-prepared_Gel_of_Minced_Shrimp_Litopenaeus_Vannamei_During_Frozen_Storage
- Yan S, Liu X, Sang Y, Tian G. Gel mechanism analysis of minced scallop (Patinopecten yessoensis) meat modified by three kinds of food colloids. Food Biosci 2024;57:103541. https://doi.org/10.1016/j.fbio.2023.103541
- Sukarno AS, Nurliyani Y, Erwanto Y, Rakhmatulloh S, Rifqi. Antibacterial and antioxidant activity of protein hydrolysate extracted from different Indonesian avian egg white. Jurnal Sain Peternakan Indonesia 2023;18:27–33. https://doi.org/10.31186/jspi.id.18.1.27-33
- Meng X, Wu D, Zhang Z, Wang H, Wu P, Xu Z, et al. An overview of factors affecting the quality of beef meatballs: processing and preservation. Food Sci Nutr 2022;10:1961–74. https://doi.org/10.1002/fsn3.2812
- Madjid SA, Arief II, Wulandari Z, Sumantri C. Physicochemical, microbiological characteristics of meatballs from a mixture of IPB D1 chicken and local Indonesian rabbit meats. Malays J Sci 2024;43:1–9. https://doi.org/10.22452/mjs.vol43no4.1
- Holinesti R, Faridah A, Insan RR, Mustika S, Tasrif N. Spent hen meatballs rendang’s chemical characteristics. In: IOP Conf Ser Earth Environ Sci. 2024;1426:012021. https://doi.org/10.1088/1755-1315/1426/1/012021
- Wong YY, Chow YL. Exploring the potential of spice‐derived phytochemicals as alternative antimicrobial agents. eFood 2024;5:e126. https://doi.org/10.1002/efd2.126.
- Tanisławczyk R, Żurek J, Rudy M, Gil M, Krajewska A, Dziki D. Effect of various thermal processing methods on the sensory, textural, and physicochemical characteristics of foal meat. Molecules 2024;29:5464. https://doi.org/10.3390/molecules29225464
- Li R, Wang C, Zhou G, Li C, Ye K. The effects of thermal treatment on the bacterial community and quality characteristics of meatballs during storage. Food Sci Nutr 2020;9:564–73. https://doi.org/10.1002/fsn3.2026
- Di MP. Antimicrobial agents and microbial ecology. AIMS Microbiol 2022;8:1–4. https://doi.org/10.3934/microbiol.2022001
- Lee J, You Y. An integrated system combining filter-assisted sample preparation and colorimetric biosensing for rapid pathogen detection in complex food matrices. Foods 2025;14:2986. https://doi.org/10.3390/foods14172986
- Khalid T, Hdaifeh A, Federighi M, Cummins E, Boué G, Guillou S, et al. Review of quantitative microbial risk assessment in poultry meat: the central position of consumer behavior. Foods 2020;9:1661. https://doi.org/10.3390/foods9111661
- Betiku E, Ogundipe TT, Kalapala T, Obe T. A mini-review on multi-hurdle control of Salmonella along poultry production continuum. Animals 2025;15:875. https://doi.org/10.3390/ani15060875
- Nahusenay H, Tola A, Sisay TT, Vipham J, Woldegiorgis AZ. Seasonal comparison of microbial hygiene indicators in raw and pasteurized milk and cottage cheese collected across the dairy value chain in three regions of Ethiopia. Foods 2023;12:4377. https://doi.org/10.3390/foods12244377
- Heuser E, Becker K, Idelevich EA. Evaluation of an automated system for the counting of microbial colonies. https://doi.org/10.1128/spectrum.00673-23
- Shaji S, Selvaraj RK, Shanmugasundaram R. Salmonella infection in poultry: a review on the pathogen and control strategies. Microorganisms 2023;11:2814. https://doi.org/10.3390/microorganisms11112814
- Barcenilla C, Cobo-Díaz JF, Puente A, Valentino V, De FF, Ercolini D, et al. In-depth characterization of food and environmental microbiomes across different meat processing plants. Microbiome 2024;12:199. https://doi.org/10.1186/s40168-024-01856-3
- Jagtap GA, Badge A, Kohale MG, Wankhade RS, Wankhade R. The role of the biosafety cabinet in preventing infection in the clinical laboratory. Cureus 2023;15:e51309. https://doi.org/10.7759/cureus.51309
- Rafiq F, Rahmawati D, Untari D, Susilo DP, Nilamsari R, Lesari SS. Monitoring of animal products entering East Kalimantan with total plate count test. J Appl Vet Sci Technol 2024;5:115–121. https://doi.org/10.20473/javest.V5.I2.2024.115-121
- K Y, A NHZ, P K, R IFM, R NKMA. Effect of different packaging conditions and temperatures on physicochemical and microbiological quality of Tualang honey-marinated chicken meat. J Meat Sci 2024;19:04. https://doi.org/10.48165/jms.2024.19.02.04
- Blanco AF, Pérez MH, Moreno TYM, Hernández JG. A photonic label-free biosensor to detect Salmonella spp. in fresh vegetables and meat. Appl Sci 2023;13:13103. https://doi.org/10.3390/app132413103
- Vinayaka AC, Ngo TA, Kant K, Engelsmann P, Dave VP, Shahbazi MA, et al. Rapid detection of Salmonella enterica in food samples by a novel approach with combination of sample concentration and direct PCR. Biosens Bioelectron 2019;129:224–30. https://doi.org/10.1016/j.bios.2018.09.078
- Paniel N, Noguer T. Detection of Salmonella in food matrices, from conventional methods to recent aptamer-sensing technologies. Foods 2019;8:371. https://doi.org/10.3390/foods8090371
- Badan Pengawas Obat dan Makanan Republik Indonesia (BPOM RI). Peraturan Badan Pengawas Obat dan Makanan Republik Indonesia Nomor 13 Tahun 2019 tentang batas cemaran mikroba dalam pangan olahan. Jakarta: BPOM RI; 2019. https://www.peraturan.go.id/files/bn751-2019.pdf
- Lemos JWJF, Marques CL, Alberto GC, Sales DOV, Gava BA, Alves DOF, et al. Microbial landscape of cooked meat products: evaluating quality and safety in vacuum-packaged sausages using culture-dependent and culture-independent methods over 1 year in a sustainable food chain. Front Microbiol 2024;15:1457819. https://doi.org/10.3389/fmicb.2024.1457819
- Fadhina M, Nuraida L, Dewanti-Hariyadi R. Sintasan spora Bacillus cereus selama pengolahan dan penyimpanan rendang. J Teknol Ind Pangan 2024;36:155–66. https://doi.org/10.6066/jtip.2025.36.2.155
- Pniewski P, Anusz K, Białobrzewski I, Puchalska M, Tracz M, Kożuszek R, et al. The influence of storage temperature and packaging technology on the durability of ready to eat preservative-free meat bars with dried plasma. Foods 2023;12:4372. https://doi.org/10.3390/foods12234372
- Nouri M, Razavi SH, Afraei M, Shafiepour M, Jahan FM. Recent trend in the shelf life extension of meat and meat products using edible packaging enriched with antimicrobial peptides (AMPs). Food Control 2025;111768. https://doi.org/10.1016/j.foodcont.2025.111768
- Desvita H, Faisal M, Mahidin, Suhendrayatna. Preservation of meatballs with edible coating of chitosan dissolved in rice hull-based liquid smoke. Heliyon 2020;6:e05228. https://doi.org/10.1016/j.heliyon.2020.e05228
- Li YX, Erhunmwunsee F, Liu M, Yang K, Zheng W, Tian J. Antimicrobial mechanisms of spice essential oils and application in food industry. Food Chem 2022;382:132312. https://doi.org/10.1016/j.foodchem.2022.132312.
- Cano C, Wei X, Etaka CA, Chaves BD. Thermal inactivation of Salmonella on chicken wings cooked in domestic convection and air fryer ovens. J Food Sci 2022;87:3611–9. https://doi.org/10.1111/1750-3841.16230
- Heymans R, Vila A, van HCAM, Jansen CCC, Castelijn GAA, Van DVM, et al. Rapid detection and differentiation of Salmonella species, Salmonella Typhimurium and Salmonella Enteritidis by multiplex quantitative PCR. PLoS One 2018;13:e0206316. https://doi.org/10.1371/journal.pone.0206316
- Wahyuni DK, Kharisma VD, Murtadlo AAA, Rahmawati CT, Syukriya AJ, Prasongsuk S, et al. The antioxidant and antimicrobial activity of ethanolic extract in roots, stems, and leaves of three commercial Cymbopogon species. BMC Complement Med Ther 2024;24:272. https://doi.org/10.1186/s12906-024-04573-4
- Asif A, Ibrahim F, Ansari A. A systematic review: effectiveness of herbs and spices as natural preservatives to enhance meat shelf life. J Health Rehabil Res 2024;4:1–7. https://doi.org/10.61919/jhrr.v4i3.1419
- Orimaye OE, Ekunseitan DA, Omaliko PC, Fasina YO. Mitigation potential of herbal extracts and constituent bioactive compounds on Salmonella in meat-type poultry. Animals 2024;14:1087. https://doi.org/10.3390/ani14071087
- Tîrziu E, Bărbălan G, Morar A, Herman V, Cristina RT, Imre K. Occurrence and antimicrobial susceptibility profile of Salmonella spp. in raw and ready to eat foods and Campylobacter spp. in retail raw chicken meat in Transylvania, Romania. Foodborne Pathog Dis 2020;17:479–84. https://doi.org/10.1089/fpd.2019.2738
- Obe T, Boltz T, Kogut M, Ricke SC, Brooks LA, Macklin K, et al. Controlling Salmonella: strategies for feed, the farm, and the processing plant. Poult Sci 2023;102:103086. https://doi.org/10.1016/j.psj.2023.103086