Optimizing Hydroponically Cultivated Kale Growth Using Nano-Formulated Organic Fertilizers: A Multivariate Evaluation Using PCA
##plugins.themes.academic_pro.article.main##
Abstract
Kale (Brassica oleracea var. acephala) cultivated in hydroponic systems commonly relies on inorganic AB Mix nutrients, which raises sustainability concerns. This study evaluated the potential of nano-technology liquid organic fertilizers (nano-POC) derived from Sargassum, Gliricidia sepium (gamal leaves) and Tithonia diversifolia as partial substitutes for AB Mix in hydroponic kale cultivation. The experiment was conducted using different substitution formulations, and plant growth responses were assessed based on morphological and biomass-related parameters. The results showed that partial substitution of AB Mix with nano-POC maintained superior growth performance compared to full organic application. The formulation P3S (50% AB Mix + 50% Sargassum nano-POC) produced the most balanced and consistent growth responses, reaching a plant height of 22.5 cm, an average of seven leaves and a dry weight of 0.17 g, and showed no significant difference from the 100% AB Mix control. In contrast, the exclusive use of nano-POC resulted in significant declines across all growth indicators. Multivariate analysis using Principal Component Analysis (PCA) confirmed the stability and consistency of the optimal treatment across growth parameters. These findings indicate that nano-POC functions effectively as a complementary nutrient source in hydroponic systems. Integrating nano-technology liquid organic fertilizers through partial substitution strategies offers a sustainable approach to reducing dependence on synthetic fertilizers while maintaining hydroponic kale productivity.
##plugins.themes.academic_pro.article.details##

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
References
- Sambo P, Nicoletto C, Giro A, Pii Y, Valentinuzzi F, Mimmo T, et al. Hydroponic solutions for soilless production systems: Issues and opportunities in a smart agriculture perspective. Front Plant Sci 2019;10:1–17. https://doi.org/10.3389/fpls.2019.00923.
- Mielcarek A, Kłobukowska K, Rodziewicz J. Water Nutrient Management in Soilless Plant Cultivation versus Sustainability. Sustainability 2024;16:1–19. https://doi.org/10.3390/su16010152.
- Fathidarehnijeh E, Nadeem M, Cheema M, Thomas R, Krishnapillai M, Galagedara L. Current perspective on nutrient solution management strategies to improve the nutrient and water use efficiency in hydroponic systems. Canadian Journal of Plant Science 2023;104:88–102. https://doi.org/10.1139/cjps-2023-0034.
- Arora PK, Tripathi S, Omar RA, Chauhan P, Sinhal VK, Singh A, et al. Next-generation fertilizers: the impact of bionanofertilizers on sustainable agriculture. Microb Cell Fact 2024;23:1–18. https://doi.org/10.1186/s12934-024-02528-5.
- Usman M, Farooq M, Wakeel A, Nawaz A, Cheema SA, Rehman H ur, et al. Nanotechnology in agriculture: Current status, challenges and future opportunities. Science of The Total Environment 2020;721. https://doi.org/10.1016/j.scitotenv.2020.137778.
- Nongbet A, Mishra AK, Mohanta YK, Mahanta S, Ray MK, Khan M, et al. Nanofertilizers: A Smart and Sustainable Attribute to Modern Agriculture. Plants 2022;11:1–20. https://doi.org/10.3390/plants11192587.
- Jakhar AM, Aziz I, Kaleri AR, Hasnain M, Haider G, Ma J, et al. Nano-fertilizers: A sustainable technology for improving crop nutrition and food security. NanoImpact 2022;27. https://doi.org/10.1016/j.impact.2022.100411.
- Saurabh K, Prakash V, Kumar A, Sonaka D, Arti G, Prem K. Enhancing sustainability in agriculture with nanofertilizers. Discover Applied Sciences 2024;6:1–23. https://doi.org/10.1007/s42452-024-06267-5.
- Utami Y, Fevria R, Vauzia, Putri ILE. The Effect of Nano Technology Liquid Organic Fertilizer on the Growth of Green Lettuce (Lactuca sativa L. var longifolia) Cultivated Hydroponically. Jurnal Biologi Tropis 2023;22:61–7. https://jurnalfkip.unram.ac.id/index.php/JBT/article/view/4718/2923
- Alneyadi KSS, Almheiri MSB, Tzortzakis N, Di Gioia F, Ahmed ZFR. Organic-based nutrient solutions for sustainable vegetable production in a zero-runoff soilless growing system. J Agric Food Res 2024;15:101035. https://doi.org/10.1016/j.jafr.2024.101035.
- Fahrurrozi F, Muktamar Z, Setyowati N, Chozin M, Sudjatmiko S. Nutrient Properties of Tithonia-enriched Liquid Organic Fertilizer as Affected by Different Types of Animal Feces and Its Effects on Fresh Weight of Loose-leaf Lettuce (Lactuva sativa L.). International Journal on Advanced Science Engineering Information Technology 2020;10:730–5. https://doi.org/10.18517/ijaseit.10.2.4748.
- Yusnaeni, Nikmah, Honin MC, Bako D. The Effectiveness of Sargassum sp as Liquid Organic Fertilizer in Increasing The Growth of Red Chillies. Indonesian Journal of Science and Education 2022;6:1–7. https://doi.org/10.31002/ijose.v6i1.129.
- Alamu EO, Adesokan M, Fawole S, Maziya-Dixon B, Mehreteab T, Chikoye D. Gliricidia sepium (Jacq.) Walp Applications for Enhancing Soil Fertility and Crop Nutritional Qualities: A Review. Forests 2023;14:1–13. https://doi.org/10.3390/f14030635.
- Fevria R, Razak A, Vauzia, Putri SD, Annisa S. The Application of Nanotechnology-Based Liquid Organic Fertilizer for Improving the Quality of Hydroponically Grown Vegetables. Journal of Applied Agricultural Science and Technology 2025;9:375–83. https://doi.org/10.55043/jaast.v9i3.398.
- Fevria R, Razak A, Heldi, Syah N, Kamal E, Edwin. Application of nanotechnology liquid organic fertilizer in sustainable hydroponic cultivation for urban food security. Science and Technology Asia 2023;28:295–304. https://doi.org/10.14456/scitechasia.2023.89.
- Cui J, Zhan X, Wang Q, Zhang D, Li D, Li X, et al. Principal Component Analysis and Cluster Analysis of Hydroponic Adaptation Potential in Different Pakchoi (Brassica campestris ssp. Chinensis) Parent Materials. Horticulturae 2025;11:1–20. https://doi.org/10.3390/horticulturae11070822.
- Crista L, Radulov I, Crista F, Imbrea F, Manea DN, Boldea M, et al. Utilizing Principal Component Analysis to Assess the Effects of Complex Foliar Fertilizers Regarding Maize (Zea mays L.) Productivity. Agriculture 2024;14:1–19. https://doi.org/10.3390/agriculture14081428.
- Palsha P Lou, Iersel MW Van, Dickson RW, Seymour L, Yelton M, Qin K, et al. Strategic Light Use Efficiency Optimization of Hydroponic Lettuce Exposed to Different Photosynthetic Photon Flux Densities. Agronomy 2024;14:1–14. https://doi.org/10.3390/agronomy14102281.
- Endoh T, Takagaki M, Suwitchayanon P, Chanseetis C, Lu N. Hydroponic Lettuce Cultivation with Organic Liquid Fertilizer: Examining Bacterial Inhibition and Phosphate Solubilization. Crops 2024;4:502–13. https://doi.org/10.3390/crops4040036.
- Chowdhury M, Samarakoon UC, Altland JE. Evaluation of hydroponic systems for organic lettuce production in controlled environment. Front Plant Sci 2024;15:1–12. https://doi.org/10.3389/fpls.2024.1401089.
- Delden SH Van, Nazarideljou MJ, Marcelis LFM. Nutrient solutions for Arabidopsis thaliana : a study on nutrient solution composition in hydroponics systems. Plant Methods 2020;16:1–14. https://doi.org/10.1186/s13007-020-00606-4.
- Sakamoto M, Suzuki T. Effect of Nutrient Solution Concentration on the Growth of Hydroponic Sweetpotato. Agronomy 2020;10:1–14. https://doi.org/10.3390/agronomy10111708.
- El-Shal RM, El-Naggar AH, El-Beshbeshy TR, Mahmoud EK, El-Kader NIA, Missaui AM, et al. Effect of Nano-Fertilizers on Alfalfa Plants Grown under Different Salt Stresses in Hydroponic System. Agriculture 2022;12:1–19. https://doi.org/10.3390/agriculture12081113.
- Cerozi B da S, Fitzsimmons K. The effect of pH on phosphorus availability and speciation in an aquaponics nutrient solution. Bioresour Technol 2016;219:778–81. https://doi.org/10.1016/j.biortech.2016.08.079.
- Wang Y, Yang T, Kim H-J. pH Dynamics in Aquaponic Systems : Implications for Plant and Fish Crop Productivity and Yield. Sustainability 2023;15:1–20. https://doi.org/10.3390/su15097137.
- Ali O, Ramsubhag A, Jayaraman J. Biostimulant properties of seaweed extracts in plants: Implications towards sustainable crop production. Plants 2021;10:1–27. https://doi.org/10.3390/plants10030531.
- Basar NU, Shahid MA, San A, Primo B, Kadyampakeni DM. Synergies between biostimulants and plant nutrients : a review of ecofriendly nutrient management in crop production. Discover Agriculture 2025;3:1–30. https://doi.org/10.1007/s44279-025-00345-x.