Effect of the Combination of the Growth Regulators and Putrescine on the Somatic Embryogenesis of Wheat (Triticum Aestivum L.) on Some Types of Explants

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Published Nov 24, 2023
https://doi.org/10.55043/jaast.v7i4.158
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Vol. 7 No. 4 (2023): Journal of Applied Agricultural Science and Technology

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Yusniwati Yusniwati
Andalas University
Ryan Budi Setiawan
Andalas University

Abstract

Plant breeding programs need to be carried out in order to improve the genetics of wheat that is able to adapt to tropical environments through hybridization, mutation induction, tissue culture, and genetic transformation.  In vitro culture through somatic embryogenesis pathways plays an important role in genetic improvement and its integration with other breeding programs can positively affect the improvement of wheat quality, quantity, and development in Indonesia. The purpose of this study was to obtain an embryogenic callus induction method from the Dewata variety using five different types of explants, namely mature Seeds, immature embryos, immature seeds, leaf,  stem, and to obtain combination of plant growth regulators and putrescine on somatic embryogenesis of wheat.   The experimental design was prepared based on a complete randomized design with a combination treatment of embryogenic callus induction media consisting of 9 levels, namely: 1 ppm 2.4- D, 1 ppm 2.4-D + 1 ppm Picloram, ppm 2.4-D + 1 mg / L Picloram, 1 ppm 2.4-D + 10-4  M Putrescine, 1 ppm 2.4-D + 1 ppm Picloram +10-4  M Putrescine, 2 ppm 2.4-D + 1 ppm Picloram + 10-4  M Putrescine, 1 ppm 2.4 D + 10-3 M Putrescine, 1 ppm 2.4 D + 1 ppm Picloram + 10-3 M Putrescine, 2 ppm 2.4 D + 1 ppm Picloram + 10-3 M Putrescine. The results showed that the media used was able to induce embryogenic callus using mature seed and immature embryo, but  immature seed and leaf were not able to produce embryogenic callus. The best media  that produced the highest percentage of embryogenic callus was 2 ppm 2.4-D + 1 ppm Picloram + 10-4  M Putrescine with as much as 85.9% in young embryo explants.

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Author Biographies

Yusniwati Yusniwati, Andalas University

Department of Agronomi

Ryan Budi Setiawan, Andalas University

Department of Agronomi

How to Cite
Yusniwati, Y., & Setiawan, R. B. . (2023). Effect of the Combination of the Growth Regulators and Putrescine on the Somatic Embryogenesis of Wheat (Triticum Aestivum L.) on Some Types of Explants. Journal of Applied Agricultural Science and Technology, 7(4), 414-423. https://doi.org/10.55043/jaast.v7i4.158

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