Effect of Ultraviolet-C Irradiation on Morphological Character Changes in Patchouli (Pogostemon cablin Benth.)

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Published Aug 27, 2024
https://doi.org/10.55043/jaast.v8i3.284
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Vol. 8 No. 3 (2024): Journal of Applied Agricultural Science and Technology

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Sindi Haryanti
Andalas University
Yusniwati Yusniwati
Andalas University
Gustian Gustian
Andalas University

Abstract

Patchouli (Pogostemon cablin Benth.) is among the top 20 essential oil-producing plants traded in the global market. This plant shows narrow genetic diversity due to non-flowering and seed production, increasing the challenges in obtaining new varieties through crossbreeding. Therefore, this research aimed to apply mutation method using ultraviolet-C irradiation combined with tissue culture to broaden the genetic diversity of patchouli. The potential of UV-C irradiation and the morphological changes occurring in the plant were explored from October to December 2023 at the Tissue Culture Laboratory, Andalas University, Padang. In addition, the experiment was arranged in a Randomized Complete Block Design (RCBD) with 10 treatment levels and 3 groups. The treatments included UV-C irradiation doses of 0 (wild type), 30, 60, 90, 120, 150, 180, 210, 240, and 270 minutes at a distance of 30 cm. Data for each patchouli plant per observation variable were presented as mean values, variance, and standard deviation and analyzed using an unpaired t-test. The results showed that exposure to ultraviolet-C radiation impacted several morphological characteristics, such as chlorosis, delayed bud development, increased bud and leaf count, as well as faster bud growth. This phenomenon shows the potential of ultraviolet-C radiation as a physical mutagenic agent.

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Author Biographies

Sindi Haryanti, Andalas University

Department of Agronomy, Faculty of Agriculture

Yusniwati Yusniwati, Andalas University

Department of Agronomy, Faculty of Agriculture

Gustian Gustian, Andalas University

Department of Agronomy, Faculty of Agriculture

How to Cite
1.
Haryanti S, Yusniwati Y, Gustian G. Effect of Ultraviolet-C Irradiation on Morphological Character Changes in Patchouli (Pogostemon cablin Benth.). J. appl. agricultural sci. technol. [Internet]. 2024Aug.27 [cited 2024Oct.15];8(3):347-58. Available from: https://www.jaast.org/index.php/jaast/article/view/284

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